This lesson introduces the structure of plant and animal cells and explains the functions of their major organelles. Learners will identify key cell components such as the cell membrane, nucleus, cytoplasm, mitochondria and ribosomes. The lesson also develops practical laboratory skills by guiding students to observe plant and animal cells under a microscope using appropriate staining techniques.
Understanding cell structure is fundamental in Biology because cells are the basic units of life and perform essential processes required for the survival of organisms.
Cell
Cell organelle
Cell membrane
Cell wall
Cytoplasm
Nucleus
Mitochondria
Ribosomes
Chloroplast
Sap vacuole
Microscopy
Staining techniques
A cell is the smallest unit of life capable of carrying out all essential biological functions.
All living organisms are made up of cells.
respiration
growth
reproduction
transport of substances
response to environmental stimuli
Cells arise from pre-existing cells through cell division.

unicellular organisms (e.g. protozoa)
multicellular organisms (e.g. plants and animals)

Animal cells consist of several structures known as organelles, each performing a specific function.
The cell surface membrane surrounds the cell.
controls movement of substances into and out of the cell
maintains the internal environment of the cell
protects the cell from external damage
The membrane is selectively permeable, meaning it allows some substances to pass while restricting others.
The cytoplasm is a jelly-like substance that fills the cell.
contains enzymes that control metabolic reactions
supports organelles
allows transport of materials within the cell
Most chemical reactions in the cell occur in the cytoplasm.
The nucleus is usually the largest organelle in the cell.
controls the activities of the cell
contains genetic material (DNA)
regulates cell division
directs protein synthesis
Because of this role, the nucleus is often described as the control centre of the cell.
Mitochondria are rod-shaped organelles responsible for aerobic respiration.
release energy from glucose
supply energy needed for cell activities such as movement, growth and active transport
Cells that require more energy contain large numbers of mitochondria.
Ribosomes are small particles found in the cytoplasm.
site of protein synthesis
produce enzymes and structural proteins required by the cell
Although small, ribosomes are essential for cell survival.

Plant cells contain most organelles found in animal cells but also possess additional structures.
The cell wall is a rigid outer layer surrounding the cell membrane.
provides structural support
maintains the shape of the cell
prevents the cell from bursting when water enters
The cell wall is mainly composed of cellulose.
Chloroplasts are green organelles found in plant cells.
contain chlorophyll
carry out photosynthesis
carbon dioxide
water
sunlight
The sap vacuole is a large fluid-filled sac.
stores water, nutrients and waste materials
maintains turgor pressure which helps keep the plant upright

These differences reflect the different functions of plants and animals.

Cells are too small to be seen with the naked eye. They are studied using microscopes.
plant cells such as onion epidermis
animal cells or protozoa
Stains are used to make cell structures easier to see.
Staining improves visibility of structures such as the nucleus and cytoplasm.

Prepare a thin specimen (e.g. onion epidermis).
Place the specimen on a microscope slide.
Add a drop of stain.
Cover with a cover slip.
Observe under low magnification.
Adjust focus to view cell structures clearly.
cell wall
cell membrane
nucleus
cytoplasm
chloroplasts
If asked to:
Identify cell structures
Label organelles clearly and accurately on diagrams or photomicrographs.
Describe functions
State the organelle and link it directly to its biological function.
Example:
“Ribosomes are the site of protein synthesis.”
preparation of slide
staining technique
use of microscope
Avoid vague statements such as “look at the cells”.
Confusing the cell membrane with the cell wall
Saying mitochondria “produce energy” instead of releasing energy through respiration
Forgetting that ribosomes synthesize proteins
Failing to describe the role of staining in microscopy
Listing cell structures without explaining their functions
Identify two structures found in both plant and animal cells.
State the function of the cell surface membrane.
Explain the function of mitochondria in a cell.
Explain two differences between plant and animal cells.
“The nucleus is the most important organelle in a cell.”
Discuss this statement.
Strong answers should correctly identify structures such as:
nucleus
cytoplasm
cell membrane
mitochondria
Incorrect answers often include plant-only structures such as chloroplasts.
identify mitochondria as the site of aerobic respiration
explain that energy is released from glucose
link energy to cell activities
The command word Explain requires linking structure to function.
High-level responses should:
recognise the nucleus controls cell activities
explain that other organelles such as mitochondria and ribosomes are also essential
A balanced judgement must be provided.